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プロテオミクスから骨格筋の性別二型
JoVE Journal
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Skeletal Muscle Gender Dimorphism from Proteomics
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プロテオミクスから骨格筋の性別二型

12,254 Views

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09:29 min

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December 14, 2011

DOI:

10.3791/3536-v

DOI:
10.3791/3536-v

•

09:29 min
December 14, 2011

•

1 Views
, , ,
1Center for Proteomics,Smith College, 2Department of Molecular Biophysics and Biochemistry,Yale University, 3Kimya Bölümü,Smith College, 4Department of Biological Sciences and Center for Proteomics,Smith College

Özet

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分離して骨格筋に発現し、最も豊富なタンパク質の同一性を決定するための方法の直接的なセット。約800スポットが10 mgの筋から二次元ゲル上に明察しているが、これは性別特異的タンパク質の発現の判定が可能になります。これらのメソッドは、ほとんどの組織で同等の結果が得られます。

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Dimova, K., Metskas, L. A., Kulp, M., Scordilis, S. P. Skeletal Muscle Gender Dimorphism from Proteomics. J. Vis. Exp. (58), e3536, doi:10.3791/3536 (2011).

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