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Skeletal Muscle Gender Dimorphism from Proteomics
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December 14, 2011
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A straight-forward set of methods to isolate and determine the identity of the most abundant proteins expressed in skeletal muscle. About 800 spots are discerned on a two-dimensional gel from 10 mg muscle; this allows for the determination of gender-specific protein expression. These methods will give equivalent results in most tissues.
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Skeletal MuscleGender DimorphismProteomicsContractile ProteinsHypertrophyAtrophyRemodelingAdaptations To StressorsEccentric ContractionDamage To MuscleRepairDegradationResynthesisGlobal ProteomeExerciseProtein ExpressionDifferential Protein ExpressionAdaptations To Damage And RepairMale MuscleFemale MuscleTwo-dimensional Gel ElectrophoresisHigh Resolution Digital Imaging
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Dimova, K., Metskas, L. A., Kulp, M., Scordilis, S. P. Skeletal Muscle Gender Dimorphism from Proteomics. J. Vis. Exp. (58), e3536, doi:10.3791/3536 (2011).
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