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Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization
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January 13, 2012
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Live-cell imaging of caspase-3 mediated apoptosis in immortalized N19-oligodendrocyte cell cultures using the NucView 488 caspase-3 substrate. This technique is applicable for programmed cell death assays in real-time in a variety of cell types and tissues.
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Cleaved Caspase-3 ActivityApoptosisImmortalized Oligodendroglial CellsLive-cell ImagingCleaveable Fluorogenic-dye SubstratesPotassium-induced Membrane DepolarizationNeurological InsultsNeuronal Population And FunctionDamaged AxonsGlial CellsAstrocytesOligodendrocytesProgrammed Cell DeathSignal Transduction CascadesTraumatic Brain InjuryCerebral IschemiaSeizuresCaspasesChronic Neurodegenerative DiseasesAlzheimer’s DiseaseHuntington’s DiseaseMultiple SclerosisNucView 488 Caspase-3 Substrate
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Smith, G. S., Voyer-Grant, J. A., Harauz, G. Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization. J. Vis. Exp. (59), e3422, doi:10.3791/3422 (2012).
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