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Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
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February 13, 2012
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1Department of Physiology and Biophysics, Confocal Microscopy and Cell Imaging Core,Robert Wood Johnson Medical School , 2Department of Physiology and Biophysics,Robert Wood Johnson Medical School , 3Muscle Biology Research Group-MUBIG Schools of Nursing & Medicine,University of Missouri-Kansas City
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The extent of store-operated Ca2+ entry (SOCE) can be monitored using fluorescent Ca2+ indicators. Mn2+ quenching of such indicators assays SOCE in cultured cells and skeletal muscle fibers. A technique allowing spatial and temporal resolution of SOCE by confocal imaging of mechanically skinned muscle fibers is also described.
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Pan, Z., Zhao, X., Brotto, M. Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber. J. Vis. Exp. (60), e3415, doi:10.3791/3415 (2012).
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