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Using Affordable LED Arrays for Photo-Stimulation of Neurons
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JoVE Journal Nörobilim
Using Affordable LED Arrays for Photo-Stimulation of Neurons

Using Affordable LED Arrays for Photo-Stimulation of Neurons

DOI:
10.3791/3379-v

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07:40 min

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November 15, 2011

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, , ,
1Laboratory for Perception and Memory,Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)

Bölümler

  • 00:05Başlık
  • 01:43Optical Calibration: Measuring LED Power
  • 04:35Applying the LED Array to Physiological Testing
  • 05:55Photo-stimulation with an LED Array
  • 07:07Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Adult-born neurons expressing ChR2 can be manipulated in slice electrophysiological preparations in order to examine their contribution towards the function of olfactory neural circuits.

Etiketler

Affordable LED ArraysPhoto-stimulationNeuronsStandard Slice ElectrophysiologyElectrical ResponsesSingle CellsOptogeneticsGenetically Targeted NeuronsChannelrhodopsin-2 (ChR2)Activation Of Neurons With LightLED-based PhotostimulationAdult-born InterneuronsOlfactory BulbOlfactory SystemViral ExpressionChR2-YFPOptical ControlLED System CalibrationSpiking ActivityFiring Pattern

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