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LentiPlex MISSIONE pool di screening Biblioteca shRNA in cellule di mammifero
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December 21, 2011
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Qui usiamo una libreria umano LentiPlex raggruppati e tradizionali metodi di sequenziamento per identificare gli obiettivi gene promuovere la sopravvivenza delle cellule. Dimostriamo come impostare e deconvolute uno schermo LentiPlex e convalidare i risultati.
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MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes
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Coussens, M. J., Corman, C., Fischer, A. L., Sago, J., Swarthout, J. MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells. J. Vis. Exp. (58), e3305, doi:10.3791/3305 (2011).
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