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检测组蛋白修饰在植物叶片
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Detection of Histone Modifications in Plant Leaves

检测组蛋白修饰在植物叶片

DOI:

07:08 min

September 23, 2011

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Bölümler

  • 00:05Başlık
  • 01:25Crosslinking of Plant Material
  • 02:25Isolation of Chromatin
  • 04:18Immunoprecipitation
  • 05:26Locus-specific DNA Quantitation
  • 05:55Benzothiadiazole (BTH) Induced Histone Acetylation on the PR2 Promoter in Arabidopsis
  • 06:20Conclusion

Özet

Otomatik Çeviri

一个可靠的和有用的方法来检测特定的植物基因组蛋白修饰的描述。该方法结合了染色质免疫沉淀(ChIP)和实时定量PCR。它允许在不同的生理过程作用,对特定的基因组蛋白修饰的检测。

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