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Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica
Bölümler
- 00:05Başlık
- 01:09Trunk Fragment Regeneration Assay
- 03:00Pharmacological Manipulation of Regeneration: Bipolarity Induced by Praziquantel
- 04:13Genetic Manipulation of Regeneration: in vivo RNAi Feeding Protocol
- 07:54A Standard RNAi Treatment Protocol and Representative Assay Results
- 09:36Conclusion
An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.
