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In vivo Electroporation of Developing Mouse Retina
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05:53 min
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June 24, 2011
DOI:
1Solomon H. Snyder Department of Neuroscience,Johns Hopkins School of Medicine, 2Department of Neurology,Johns Hopkins School of Medicine, 3Department of Ophthalmology,Johns Hopkins School of Medicine, 4Center for High-Throughput Biology,Johns Hopkins School of Medicine, 5Institute for Cell Engineering,Johns Hopkins School of Medicine
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A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.
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de Melo, J., Blackshaw, S. In vivo Electroporation of Developing Mouse Retina. J. Vis. Exp. (52), e2847, doi:10.3791/2847 (2011).
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