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Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
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June 22, 2011
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A method is described for the preparation of single living photoreceptor cells from different vertebrate species for fluorescence imaging. The method can be used to image the fluorescence of endogenous fluorophores, such as NADH or vitamin A, or that of exogenously added fluorescent dyes sensitive to Ca2+ or other factors.
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Boyer, N. P., Chen, C., Koutalos, Y. Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging. J. Vis. Exp. (52), e2789, doi:10.3791/2789 (2011).
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