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Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging

Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging

DOI:
10.3791/2789-v

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11:24 min

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June 22, 2011

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, ,
1Storm Eye Institute,Medical University of South Carolina

'Bölümler'

  • 00:05'Başlık'
  • 01:24Prep Work: Dished, Chambers, Blades and Solutions
  • 02:47Isolation of Retinas
  • 04:49Isolation of Single Photoreceptor Cells
  • 06:17Fluorescence Imaging
  • 09:29Morphology and Health of Isolated Photoreceptor Cells
  • 10:53Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

A method is described for the preparation of single living photoreceptor cells from different vertebrate species for fluorescence imaging. The method can be used to image the fluorescence of endogenous fluorophores, such as NADH or vitamin A, or that of exogenously added fluorescent dyes sensitive to Ca2+ or other factors.

'Etiketler'

Living Isolated Vertebrate Photoreceptor CellsFluorescence ImagingPhototransductionRod PhotoreceptorsCone PhotoreceptorsOuter SegmentVisual PigmentChromophoreOpsinPhoton AbsorptionPhotoisomerizationConformational ChangeCascade Of ReactionsMembrane PotentialElectrical SignalRecovery ProcessDeactivation Of IntermediatesCa2+ ModulationAdaptation To Background LightRegeneration Of Visual Pigment

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