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A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.' 'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
'Bölümler'
- 00:05'Başlık'
- 01:49Production and Purification of sGMRalpha
- 03:23Biotinylation of Recombinant GM-CSF
- 04:09A Cell-free GMCSF Receptor-ligand-binding Assay
- 05:22From Bench to Bedside
- 07:00Conclusion
We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.
'Etiketler'
Cell-free Assay SystemNeutralizing CapacityGM-CSF AntibodyRecombinant Soluble GM-CSF ReceptorAutoimmune Pulmonary Alveolar Proteinosis (PAP)Disease SeverityGM-CSF BioactivityNeutralizing Capacity AssessmentGrowth InhibitionHuman Bone Marrow CellsTF-1 CellsBioassay SystemCell-free Receptor-binding AssayTransgenic Silkworm TechnologyRecombinant SGMRαSilk ThreadsCocoons
