/
/
Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
Bölümler
- 00:05Başlık
- 01:15Bacterial Challenge and Tissue Sampling
- 02:29Dewaxing the Sections and Immunohistochemistry
- 04:26Representative Fluorescent Images
- 04:48Conclusion
Here we describe a procedure allowing the labeling of Edwardsiella ictaluri in situ in histological sections from channel catfish Ictalurus punctatus using indirect immunohistochemistry with monoclonal antibodies Ed9 as a primary, and fluorescent FitC labeled antibodies as a secondary. This allowed for the detection of the bacterium using fluorescent microscopy.
Etiketler
Fluorescent Immuno-ChemistryDetectionEdwardsiella IctaluriChannel CatfishIctalurus PunctatusMethod DevelopmentIn Situ VisualizationBacteriaHistological SectionsBacterial LocalizationPlate CountsRadiometric LabeledBioluminescent BacteriaGross Organ LevelInfection CycleVirulence FactorsInteraction With HostSalmonella TyphiTaxonomic FamilyTechnique DescriptionIndirect Immuno-histochemistryMonoclonal Ed9 AntibodyBlocking SerumParaffin Embedded Histological SectionsPrimary AntibodySecondary AntibodiesFitC Labeled Secondary Antibodies
