Dergi
/
Nörobilim
/
Transcriptome Analysis of Single Cells
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

DOI:
10.3791/2634-v

•

07:27 min

•

April 25, 2011

•

, ,
1Department of Pharmacology,University of Pennsylvania, 2The Penn Genome Frontiers Institute,University of Pennsylvania

Bölümler

  • 00:05Başlık
  • 01:11Preparing Culture and Pipettes
  • 01:53Harvesting Cells
  • 05:04Saving the Cell
  • 06:07Representative Results for Single Cell Harvest and aRNA Analysis
  • 07:08Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

In this article we describe a simple method for the harvesting of single cells from rat primary neuronal cultures and subsequent transcriptome analysis using aRNA amplification. This approach is generalizable to any cell type.

Etiketler

Transcriptome AnalysisSingle CellsGene Expression AnalysisHeterogeneous PopulationsCell CultureHippocampusMRNA ProfilesMolecular DifferencesCell PopulationsHarvesting CellsElektrofizyolojiEppendorf TubeMolecular Biology TechniquesARNA AmplificationDownstream ApplicationReverse-transcriptionReal-time Polymerase Chain Reaction (PCR)Linear AmplificationPolyadenylated RNA

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Peering at Brain Polysomes with Atomic Force Microscopy

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish

Read Article