This content is Free Access.
Myo-mechanical Analysis of Isolated Skeletal Muscle
26,349 Views
•
08:42 min
•
February 22, 2011
DOI:
1Cardiovascular Research Institute,University of California San Francisco, 2Pediatri Anabilim Dalı,University of California San Francisco, 3Biyoloji Bölümü,San Francisco State University, 4Tıp Fakültesi,University of California San Francisco , 5Eli and Edythe Broad Center of Regeneration Medicine & Stem Cell Research,University of California San Francisco
Özet
Automatically generated
To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.
Explore More Videos
Myo-mechanical AnalysisIsolated Skeletal MuscleTherapeutic InterventionsMuscle DiseaseQuantitative MethodsForce GenerationFatigabilityHindlimb MuscleExplanted MuscleMuscle Strip MyographMaximal Twitch TensionTetanic TensionContraction TimeHalf-relaxation TimeSquare Pulse StimulatorSpecific Twitch TensionSpecific Tetanic TensionMuscle Cross-sectional AreaTwitch-to-tetanic Tension RatioForce-frequency Relationship CurveLow Frequency Fatigue CurveComparison Between Therapeutic InterventionsMouse Models Of Muscle DiseaseGenetic ModificationMuscle Function
Read Article
Cite this Article
Oishi, P. E., Cholsiripunlert, S., Gong, W., Baker, A. J., Bernstein, H. S. Myo-mechanical Analysis of Isolated Skeletal Muscle. J. Vis. Exp. (48), e2582, doi:10.3791/2582 (2011).
Copy