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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

DOI:
10.3791/2540-v

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10:44 min

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February 02, 2011

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, , ,
1Division of Pediatrics,MD Anderson Cancer Center – University of Texas

Bölümler

  • 00:05Başlık
  • 01:13Introduction
  • 01:40Isolation of Cell Fractions from Buffy Coat
  • 03:34NK Cell Expansion
  • 06:34NK Cell Cytotoxicity Assay
  • 08:33Representative Results
  • 09:55Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we describe a method to efficiently expand and purify large numbers of human NK cells and assess their function.

Etiketler

ExpansionPurificationFunctional AssessmentHumanPeripheral BloodNK CellsNatural Killer CellsImmune SurveillanceInfectious MicroorganismsTumorsLimited AvailabilityIn VitroNK Cell ImmunotherapyMethodEfficiently ExpandVast QuantitiesFunctional NK CellsEx VivoK562 Cells Expressing Membrane-bound IL21Artificial Antigen-presenting Cell (aAPC)Cell ProductSteady-state LeukapheresisDonorDepletion Of T CellsPositive Selection Of NK CellsIL-2 Overnight ActivationSmall Numbers Of NK Cells Delivered In Clinical TrialsInability To Propagate NK Cells In VitroGenerating Sufficient Cell Numbers

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