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Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
Bölümler
- 00:05Başlık
- 01:51Preparing Worm Samples for Metabolite Quantitation
- 04:37Processing Neutralized Samples for Amino Acid and Organic Acid Analysis by GC/MS
- 07:11Monitoring Isotopic Utilization in Worms on Plates by Tracing Labeled Carbon in Atmospheric Carbon Dioxide
- 08:39Results of Stable Isotopic Profiling of Intermediary Metabolism and Metabolic Flux
- 11:40Conclusion
Stable isotopic profiling by gas chromatography mass spectrometric analysis of intermediary metabolic flux is described in the nematode, Caenorhabditis elegans. Methods are detailed for assessing isotopic enrichment in carbon dioxide, organic acids, and amino acids following isotope exposure either during development on agar plates or during adulthood in liquid culture.
Etiketler
Stable Isotopic ProfilingIntermediary MetabolismMetabolic FluxCaenorhabditis ElegansNematode Growth Media Agar PlatesPharmacologic TreatmentsHigh Performance Liquid Chromatography (HPLC)Mass SpectrometryCarbon DioxideGlycolysisPyruvate MetabolismTricarboxylic Acid CycleIsotope Exposure TimeBacterial Clearing ProtocolsWorm Disruption Methods
