Differentiation of Neural Progenitor Cells into Neurons

Seed about 500,000 mouse embryonic stem cell derivatives in 2 milliliters of basal differentiation medium per well onto the 0.1% gelatin-coated plates. Randomly divide the derivatives into three groups in order to test three phase II differentiation protocols. Incubate the plate for six hours. Then, wash the cells twice with 2 milliliters of PBS and add 2 milliliters of basal differentiation medium, N2 B27 medium 1, or N2 B27 medium 2 to each well, depending on the protocol. Place the plates into the incubator, and allow the cells to differentiate for another 10 days, changing the corresponding medium every two days.