Evaluating the Anti-Tumor Efficacy of a Bacterial Immunomodulatory Protein in a Murine Model

At least three days after injecting the MB-49 cells, after anesthetizing the animal, catheterize as just demonstrated and using a 1-milliliter syringe with 200 to 300 microliters of sterile PBS, wash the residual urine from the bladder, aspirating all the liquid.

Inject 50 micrograms of Helicobacter pylori neutrophil-activating protein or HPNAP, per 150 microliters of PBS into the bladder, leaving the syringe attached to the catheter to avoid leakage. After an hour, gently remove the catheter from the urethra and place the mouse in a cage under a heating lamp until it awakens.