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siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells

siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells

DEŞİFRE METNİ

Take two electroporation cuvettes. One cuvette contains a small interfering RNA, or siRNA, designed to inhibit the production of FIP200, an autophagy-inducing protein.

The second cuvette contains a scrambled siRNA as a control that does not inhibit FIP200 production.

Add immature dendritic cells, or iDCs; electroporate for the cellular entry of siRNA and then plate the cells.

The internalized siRNA binds to an RNA-inducing silencing complex.

The complex with the FIP200-specific siRNA cleaves its target mRNA, inhibiting FIP200 production, while the control siRNA-treated iDCs produce FIP200.

Harvest the cells and add the herpes simplex virus. Upon viral envelope-cell membrane fusion, the released DNA enters the nucleus and leads to the production of viral proteins and nucleocapsids.

Viral proteins disrupt nuclear lamins. FIP200 forms a complex that triggers a signaling cascade, inducing autophagy of the disrupted lamins to target them for degradation and facilitating the escape of viral nucleocapsids into the cytoplasm.

siRNA-mediated inhibition of FIP200 production restricts lamin autophagy, preventing nucleocapsid escape.

The cells are ready to assess siRNA-induced autophagy inhibition.

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