Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
Immunolocalization of Arabinogalactan Proteins and Pectins in Plant Tissue

Immunolocalization of Arabinogalactan Proteins and Pectins in Plant Tissue

DEŞİFRE METNİ

Take a slide with reaction wells containing thin sections of different plant tissues. The tissues are fixed and embedded inside a resin for immunostaining.

Place the slide inside a dark and humid incubation chamber to prevent light exposure and reagent evaporation during subsequent steps.

Introduce a blocking solution to prevent non-specific antibody interaction.

Wash to remove excess blocking solution, then add antigen-specific primary antibodies and incubate.

The antibodies bind to their target antigens, namely arabinogalactan proteins and pectins in the cell wall.

Introduce fluorophore-conjugated secondary antibodies, which bind to the primary antibodies.

Wash to remove the unbound antibodies, and apply a fluorescent dye that binds to cellulose, labeling the cell wall.

Add a mounting medium and seal with a coverslip.

Under a microscope, fluorescence from the dye and the bound antibodies aid in visualizing cellulose in plant cell walls colocalized with arabinogalactan proteins or pectin, exhibiting their tissue-specific distribution.

İlgili Videolar

Read Article