Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
The Effects of Shear Stress on Platelets Treated with Antibodies Against Mechanosensory Receptors

The Effects of Shear Stress on Platelets Treated with Antibodies Against Mechanosensory Receptors

DEŞİFRE METNİ

Begin with a multi-well plate containing platelet-rich plasma. Platelets express surface mechanoreceptor complexes — responsive to mechanical stimuli.

The mechanoreceptor complex contains the extracellular ligand-binding domain and macro-glycopeptide region linked to the mechanosensory domain.

Introduce anti-mechanoreceptor antibodies, cross-linking with the ligand-binding domains of the receptors on opposing platelets.

Place a droplet of the antibody-treated plasma suspension between the assemblies of the viscometer.

Apply shear stress, simulating mechanical force, pulling the antibody-bound receptors away from the platelets, leading to macro-glycopeptide region dissociation from the mechanosensory domain.

This triggers a conformational change in the mechanosensory domain, activating platelets and resulting in the expression of surface markers.

Transfer the sheared platelet suspension to a new well within the multi-well plate.

Introduce a cocktail of fluorophore-labeled marker-specific molecules interacting with corresponding platelet surface markers and fix the cells with a fixative solution.

In flow cytometry, distinct fluorescence signals correlate with the effects of shear stress on antibody-treated platelets.

İlgili Videolar

Read Article