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Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
JoVE Journal
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JoVE Journal Nörobilim
Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

DOI:
10.3791/2184-v

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08:37 min

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November 18, 2010

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,
1Biyomühendislik,University of California, San Diego, 2La Jolla Bioengineering Institute

'Bölümler'

  • 00:00'Başlık'
  • 02:05Preparations for Intravital Microscopy
  • 03:34Measuring Vessel Diameter and RBC Velocity by Intravital Microscopy
  • 06:13Perfusion Assessment and Analysis of Leukocyte Adherence by Intravital Microscopy
  • 07:08Representative Results of Intravital Microscopy
  • 08:03Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Intravital microscopy to follow temporal and spatial hemodynamic and inflammatory events in the pial microcirculation.

'Etiketler'

Intravital MicroscopyMouse BrainMicrocirculationClosed Cranial WindowAcute ConditionsChronic ConditionsPhysiological ConditionsPathophysiological ConditionsIn Vivo Fluorescence MicroscopyPial MicrocirculationReal-time RecordingEpi IlluminationFluorescence IlluminationVessel DiametersRed Blood Cell VelocitiesRBC Velocity MeasurementCross-correlationFluorescent-labeled ErythrocytesLeukocyte AdherencePlatelet AdherencePerfusion AssessmentVascular Leakage AssessmentFluorescence-labeled MarkersAlbumin-FITC MarkerAnti-CD45-TxR AntibodiesVideo RecordingPlasmodium Berghei ANKA InfectionMicrocirculatory Dysfunctions

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