Dergi
/
Biyoloji
/
Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

DOI:
10.3791/2144-v

•

08:19 min

•

September 04, 2010

•

,
1Kimya Bölümü,George Washington Üniversitesi

Bölümler

  • 00:00Başlık
  • 01:06Introduction
  • 01:34Visualization System
  • 03:27Electrospray Setup
  • 04:27Sample Preparation
  • 05:17Acquisition of Mass Spectra
  • 06:46Representative Results
  • 07:49Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Single cell analysis is performed by mass spectrometry on plant and animal cells at atmospheric pressure by using a sharpened optical fiber to sample the cells for laser ablation electrospray ionization (LAESI) mass spectrometry.

Etiketler

Single CellsMass SpectrometryAtmospheric PressureBiochemical AnalysisCell MetabolismCell CycleAdaptationDisease StatesHeterogeneous Biochemical MakeupSample PreparationAmbient Ionization MethodsMid Infrared Laser AblationBiofluidsLAESIAP IR-MALDI

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

MALDI Sample Preparation: the Ultra Thin Layer Method

Analyzing Large Protein Complexes by Structural Mass Spectrometry

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels

Read Article