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Intraperitoneal Injection into Adult Zebrafish
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Intraperitoneal Injection into Adult Zebrafish

Intraperitoneal Injection into Adult Zebrafish

DOI:
10.3791/2126-v

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09:19 min

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August 30, 2010

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, , ,
1Department of Organismal Biology and Anatomy,The University of Chicago, 2Committee on Molecular Metabolism and Nutrition,The University of Chicago, 3Tıp Fakültesi,The University of Chicago

Bölümler

  • 00:00Başlık
  • 00:54Introduction
  • 01:19Pre-injection Preparations
  • 05:34Cold-water Anesthesia, Injection, and Recovery
  • 07:56Blood Glucose Levels After 72 Hour Fast and Glucose Injection
  • 08:41Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We demonstrate intraperitoneal injection into adult zebrafish. We use a 10 μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III. This demonstration includes the use of cold water as an anesthetic.

Etiketler

Intraperitoneal InjectionAdult ZebrafishReagent AbsorptionMetabolic StudiesIP InjectionAbdominal CavityPelvic GirdleVeterinary MethodsZero MortalityBleeding At Injection SiteGentle HandlingFastingWeighingAnesthetizingRecoveryStress MinimizationSmall Injection Volume35G NeedleCortland Salt SolutionOsmotically BalancedFreshwater FishAeration Of GillsSurgical Plane Of Anesthesia

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