Modeling Photothrombotic Stroke in Mouse Model: A Laser Illumination Technique Through Mouse Skull Following Photosensitive Dye Administration to Induce Photothrombosis

Measure the body weight of the mouse to adjust the dose of Rose Bengal to be injected. Set the associated feedback-controlled heating pad to maintain the mouse's body temperature. Once the mouse is completely anesthetized and fixed in the stereotactic frame, gently insert the rectal probe to monitor the body temperature of the mouse throughout the surgical procedure. Apply the dexpanthenol eye ointment to both eyes, and clean the skin and the surrounding fur with a disinfecting agent.

Use scissors to make a single 2.0 to 2.5-centimeter longitudinal incision, and retract to expose the skull. Use cotton to gently remove the periosteum, and locate the coronal sutures. Wear protective glasses while switching on the 561-nanometer laser, and mark the bregma +3 millimeter to the left. Then, switch off the laser, and hook the sticker with a 4-millimeter diameter hole in the marked coordinates.

Inject the mouse intraperitoneally with Rose Bengal. Place the laser beam at a 4 to 5-centimeter distance from the skull. Switch on the laser, and illuminate the skull for 20 minutes. Rehydrate the skull by applying two drops of 0.9% saline. After suturing the wound, place the mouse in a recovery chamber at 37 degrees Celsius to recover from anesthesia. After one hour, place the mouse back in the cage in a temperature-controlled room.