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Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation
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JoVE Journal Nörobilim
Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation

Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation

DOI:
10.3791/2060-v

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11:12 min

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October 25, 2010

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,
1Neurobiology and Anatomy,University of Rochester

'Bölümler'

  • 00:00'Başlık'
  • 01:04Introduction
  • 01:26Thinned-skull Preparation
  • 05:08Laser Scanning Two-photon Microscopy
  • 07:32Chronic Re-imaging
  • 09:48Representative Results
  • 10:41Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

In this video and supplemental material, we show a protocol for chronic in vivo imaging of the intact brain using a thinned-skull preparation.

'Etiketler'

Chronic ImagingMouse Visual CortexThinned-skull PreparationIn Vivo ImagingTwo-photon Laser Scanning MicroscopyLiving CellsNeuronal ProcessesIntact BrainDendritic SpinesPostsynaptic Excitatory SitesCNSBrain PlasticitySynapsesCircuit RemodelingFine Cortical StructuresProtocolMinimally Invasive Approach

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