Generating Renal Cell Carcinoma Model: A Protocol to Develop Orthotopic RCC Murine Model by Intrarenal Implantation of Cancer Cells
DEŞİFRE METNİ
The implantation of renal cell carcinoma, or RCC, cells from a donor mouse to an immunocompromised recipient mouse's kidney leads to the development of an orthotopic RCC model.
To develop an orthotopic RCC model, begin by placing an anesthetized immunodeficient mouse in the prone position on a dissection board. Now, use a scalpel to make a surgical cut on the left flank of the mouse, keeping the underlying peritoneum intact.
Palpate the mouse by applying gentle pressure from the bottom. This step helps visualize the kidney through the translucent peritoneum. Next, take a syringe containing RCC cell suspension in an appropriate medium. Using a needle, inject the cell suspension into the kidney without damaging the peritoneum.
Retract the needle and seal the incision site using a tissue adhesive. Allow the mouse to recover and monitor the tumor growth at desired intervals. Initially, the injected renal cancer cells get implanted into the kidney. These cells then proliferate to form tumors.
Subsequently, a few cells may detach from the primary tumor and disseminate within the peritoneal cavity. These migrating tumor cells can invade the lung tissue and metastasize to form secondary tumors.
