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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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Establishment and Passaging of Small Intestine Organoids

JoVE Journal
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JoVE Journal Biyoloji

Establishment and Passaging of Small Intestine Organoids

Establishment and Passaging of Small Intestine Organoids

DOI:

10.3791/200273-v

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05:32 min

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June 23, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Genetik Bölümü,Yale School of Medicine, ²Pediatri Anabilim Dalı,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

Etiketler

Intestinal Organoids Small Intestine Rat Dissection Intestinal Fragment Mesentery PBS EDTA Epithelium Villi Crypts Cell Culture

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