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T波离子迁移质谱蛋白复合物分析的基本实验程序

JoVE Journal
Biyoloji

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JoVE Journal Biyoloji

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

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T波离子迁移质谱蛋白复合物分析的基本实验程序

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16:40 min

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July 31, 2010

DOI:

DOI:

•

16:40 min

July 31, 2010

•

10 Views

Izhak Michaelevski¹, Noam Kirshenbaum¹, Michal Sharon¹

¹Department of Biological Chemistry,Weizmann Institute of Science

Özet

离子迁移质谱是一个新兴的技术，分离离子气相，他们的碰撞截面和质量的基础上。该方法提供了对整体的拓扑结构和蛋白质复合物的形状的三维信息。在这里，我们勾勒出一个仪器设置和优化，校准漂移的时间，和数据解释的基本程序。

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