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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
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July 31, 2010
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Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.
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T-wave Ion Mobility-mass SpectrometryProtein Complex AnalysisIon MobilityElectric FieldCollision Cross-sectionIM SpectrometryMass SpectrometryTraveling-wave Synapt Ion Mobility Mass SpectrometerSample SeparationThree-dimensional SpectrumSpectral OverlapResolution Of Heterogeneous ComplexesDrift Time MeasurementsStructural InformationCalibration Curve
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Michaelevski, I., Kirshenbaum, N., Sharon, M. T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis. J. Vis. Exp. (41), e1985, doi:10.3791/1985 (2010).
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