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Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies
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November 16, 2009
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We present various ways to monitor heart function in the larva of Drosophila for assessing questions dealing with the function of gap junctions, ion channel mutations, modulation of pacemaker activity and pharmacological studies.
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MonitoringHeart FunctionLarval Drosophila MelanogasterPhysiological StudiesCardiac FunctionHeart RateUnrestrained LarvaeRestrained LarvaeEnvironment ControlDissected LarvaeInternal Organs RemovalBathe Heart In CompoundsExposed HeartMembrane PotentialsIonic CurrentsMyocytesElectrical ConductionHeart TubeAdvantagesDisadvantagesFuture ExperimentsLarval Heart PreparationDrosophila Skeletal NMJIntracellular Calcium RegulationCellular FunctionAction PotentialsMyocytesSpeciesIonic DysfunctionsGenes Responsible For Diseases In Mammals
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Cooper, A. S., Rymond, K. E., Ward, M. A., Bocook, E. L., Cooper, R. L. Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies. J. Vis. Exp. (33), e1596, doi:10.3791/1596 (2009).
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