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Methods for the Study of the Zebrafish Maxillary Barbel
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JoVE Journal Biyoloji
Methods for the Study of the Zebrafish Maxillary Barbel

Methods for the Study of the Zebrafish Maxillary Barbel

12,197 Views

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09:30 min

•

November 23, 2009

DOI:

10.3791/1558-v

DOI:
10.3791/1558-v

•

09:30 min
November 23, 2009

•

4 Views
,
1Department of Biological Sciences,DePaul University, 2Children’s Memorial Research Center, Department of Pediatrics,Northwestern University Feinberg School of Medicine

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The zebrafish maxillary barbel is an integumentary sense organ containing ectodermal, mesodermal and neural crest derivatives. Importantly, the adult barbel can regenerate after proximal amputation. This video introduces maxillary barbel development and demonstrates a surgical protocol to induce regeneration, followed by collection, embedding and downstream imaging of barbel specimens.

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ZebrafishMaxillary BarbelStudy MethodsSkin Sensory AppendagesEctodermal CellsMesodermal CellsNeural Crest CellsVisualizationDevelopmentMaintenanceLife CycleSurgical ProtocolRegeneration InductionAmputation PlaneRegenerative GrowthBarbel AnalysisDNA Electrophoresis

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Cite this Article

LeClair, E. E., Topczewski, J. Methods for the Study of the Zebrafish Maxillary Barbel. J. Vis. Exp. (33), e1558, doi:10.3791/1558 (2009).

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