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Un metodo HPLC lectina per arricchire Peptidi selettivamente glicosilata da campioni biologici complessi
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
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Un metodo HPLC lectina per arricchire Peptidi selettivamente glicosilata da campioni biologici complessi

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20:23 min

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October 01, 2009

DOI:

10.3791/1398-v

DOI:
10.3791/1398-v

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20:23 min
October 01, 2009

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1Obstetrics, Gynecology and Reproductive Sciences,University of California, San Francisco – UCSF, 2Buck Institute for Age Research, 3Kimya Bölümü,Purdue Üniversitesi

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Lectina coniugato perline POROS sono stati impiegati per HPLC. Standard glicopeptide serviti come controlli positivi e negativi. MARS-14 impoverito, tripsina-digerito plasmatiche umane è stato cromatografato e flow-through (FT) e le frazioni legato raccolti per analisi ESI-LC-MS/MS. Glicopeptidi sono stati arricchiti nella frazione legata, rispetto a FT.

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Johansen, E., Schilling, B., Lerch, M., Niles, R. K., Liu, H., Li, B., Allen, S., Hall, S. C., Witkowska, H. E., Regnier, F. E., Gibson, B. W., Fisher, S. J., Drake, P. M. A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples. J. Vis. Exp. (32), e1398, doi:10.3791/1398 (2009).

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