High-throughput Saccharification Assay for Lignocellulosic Materials

1. Preparation of the samples

We normally work with stems from either herbaceous or woody plants. In the case of herbaceous materials we grow the plants to maturity, and after seed development we collect dry stems once senescence is complete. The stems are chopped into 4 mm segments and placed in 2 ml vials with three milling balls. In the case of woody materials, the samples are ground to coarse sawdust with a course wood file and then placed in a ball mill for further processing.

The samples are placed in a rack within the grinding/formatting station (Figure 1). This station sequentially grinds, mixes, and weighs each sample. The number of repetitions needed per sample is established by testing the ground material in a series of preliminary experiments where the intrinsic variability for a certain particle size is determined. The 2 mL vials are pierced at the bottom and the materials are dispensed by vibration of the vial over the selected well. The vibrating arm is controlled by a feedback from the balance allowing for the accurate dispensing of the powder. The robot dispenses 4 mg/well in a standard analysis and 4 repetitions are needed in most the cases. This allows 20 plant samples/plate to be analysed.

2. Pretreatment

Once the samples are formatted, the 96-well plates are processed by an automated liquid handling system (Figure 2). Here a mild pretreatment is performed on the plant materials by adding 350 μL of acid or alkaline solutions and heating the plate on an adapted block. To avoid evaporation during the analysis, the 96 well plates are sealed with a silicone matt. The temperature and time of the pretreatment can be modified according to the materials being studied. The maximum temperature, however, is 100 °C. An alternative procedure for testing harsher pretreatments is to pretreat the materials off line and eliminate the pretreatmet from the process.

3. Hydrolysis

1. After the materials are pretreated, the robot automatically removes the pretreatment solution, and washes, all the wells, 5 times with 850 μL 25 mM Acetate Buffer. The rinses are carried out by adding buffer to the pretreatment solution, and subsequently removing 50% of the total volume after allowing the solids in the sample to settle. The height of the aspiration is set at half of the liquid mass to avoid aspirating solids from the bottom of the well; there is negligible loss of solids using this approach. After these washes the pH in the well is 4.5 and the material is ready for enzymatic hydrolysis.
2. An enzyme mixture containing a solution of cellulases and hemicellulases is added by the robot. Into each well, 850 μL of enzyme mixture is dispensed and the plate is moved into a 50 °C shaking incubator. The standard hydrolysis is performed for 8 h, but this time can be adapted to the purpose of the experiment. The standard enzyme loading used for grasses is 7 FPU/g of material.

4. Detection of reducing sugars

Determination of reducing sugars released after hydrolysis was performed using a modified 3-methyl-2-benzothiazolinone hydrozone (MTBH) method³. MBTH was selected as the most suitable method because it was the easiest to automate and the least susceptible to interference from compounds such as proteins. We modified this highly sensitive method for use on the robotic platform so that it could accurately quantify sugars at the concentrations present in the biomass hydrolysates, with a final volume of 250 μL which is suitable for a standard optical 96 well plate. All the steps below are performed automatically and three independent determinations of the reducing sugars were carried out for each saccharification reaction.

1. 30 μL of hydrolyzate was taken from the deep well plate and mixed with 45 μL of 25 mM Sodium Acetate buffer in a skirted PCR 96 well plate.
2. 25 μL of 1N NaOH and 50 μl of a solution containing 0.43 mg/ml MBTH and 0.14 mg/ml DTT were added.
3. After mixing, the PCR plate was heated at 60°C for 20 min in a thermocycler or similar device that delivers exactly the same amount of heat to all 96 wells.
4. The resulting reaction was transferred to an optical plate.
5. Add 100 μl of oxidising reagent (0.2% FeNH4(SO4)2, 0.2% sulfamic acid and 0.1% HCl). Mix well and leave to develop for at least 1 h.
6. Each plate should also contain standard reactions of 50 nmol, 100 nmol and 150 nmol glucose. The optical plates are read at 620 nm.

5. Representative Results:

Examples of different types of analyses are presented in figure 3 and 4. All the results were obtained by using the automated platform. Figure 3 represents the increase in reducing sugars released by 8 h incubations from ground poplar with increasing amounts of cellulolytic enzymes. Figure 4 presents the effects of acid and alkaline pretreatment on poplar samples. The NaOH pretreatment is more efficient that the diluted H2SO4 pretreatment in facilitating the release of sugars during hydrolysis. The amount of sugars measured after hydrolysis decreases when the pretreatment is performed using NaOH concentrations higher than 1M.

Figure 1. Robotic station for grinding and 96 well formatting of biomass

Figure 2. Liquid handling station for the High Throughput saccharification analysis

Figure 3. Effect of different enzyme loadings on the release of reducing sugar equivalents from poplar samples

Figure 4. Effect of different pretreatments on the saccharification of poplar samples. A. Sugars released after pretreament with different percentages of H2SO4 at 90°C for 30 minutes. B. Sugars released after NaOH pretreatments at the same temperature and time than the acid pretreatment.