Intranasal Immunization with BCG in a Mouse Model

Published: May 31, 2024

Abstract

Source: Uranga, S. et al., Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice. J. Vis. Exp. (2016)

This video demonstrates intranasal BCG immunization in mice, activating both innate and adaptive immune responses, leading to the development of lung-resident memory T cells and effector B cells, thus enhancing protection against Mycobacterium infections.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Quantified Glycerol Stocks of Bacillus Calmette-Guerin (BCG) Danish and Mycobacterium tuberculosis H37Rv NOTE: All the protocols described were performed under bio-safety level-3 (BSL3) conditions. Thaw frozen glycerol stocks of BCG Danish or …

Açıklamalar

The authors have nothing to disclose.

Materials

Middlebrook 7H9 broth BD 271310
Middlebrook ADC Enrichment BD 211887
Tween 80 Scharlau TW00800250
3-mm diameter Glass Beads Scharlau 038-138003
Middlebrook 7H10 Agar BD 262710
1-ml syringe 26GA 0.45×10 mm BD 301358
C tubes Miltenyi Biotec 130-093-237
M tubes Miltenyi Biotec 130-093-236
LACHRYMAL OLIVE LUER LOCK 0.60 x 30 mm. 23G x 1 1/4" UNIMED 27.134 Used as trachea cannula for BAL
Albumin, from bovine serum Sigma A4503

Etiketler

This article has been published
Video Coming Soon
Keep me updated:

.

Bu Makaleden Alıntı Yapın
Intranasal Immunization with BCG in a Mouse Model. J. Vis. Exp. (Pending Publication), e22249, doi: (2024).

View Video