Candida albicans Biofilm Formation in an In Vivo Mouse Model

Published: November 30, 2023

Abstract

Source: Kucharíková, S., et al.  Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging. J. Vis. Exp. (2015).

This video demonstrates the development of an in vivo mouse model to study the biofilm formation of Candida albicans in subcutaneous tissue. The experiment involves implanting Candida-infected devices in the animal's back region for biofilm development.

Protocol

1. C. albicans Growth Twenty-four hours before the initiation of the animal experiment, prepare yeast extract peptone dextrose, YPD plates by adding 10 g of yeast extract granulated, 20 g of bacteriological peptone, and 15 g of granulated agar. Makeup volume to 900 ml with Milli-Q water and autoclave. Add 50 ml of sterile 40% glucose. Mix thoroughly and pour into Petri dishes. Leave agar plates to cool and solidify. NOTE: In this study, use two strai…

Representative Results

Figure 1: Major steps during the animal surgery. (A) The procedure of catheter implant. (1) Place the anesthetized animal on a warm pad containing paper tissue and apply the ophthalmic ointment to the eyes. (2) Shave the lower part of the back and disinfect. (3) Create two small (approx. 0.5 cm) incisions through the skin on the left and on the right side of the back. Create a subcutaneous tunne…

Açıklamalar

The authors have nothing to disclose.

Materials

Yeast extract granulated Merck MERC1.03753.0500
Bacteriological peptone Oxoid LP037B
Agar granulated Difco 214530
Phosphate buffered saline Prepared in the laboratory for 1L of 10x PBS: 80 g NaCl, 2 g KCl, 14.4 g Na2HPO4, 2.4 g KH2PO4
RPMI1640 with L-glutamine and without sodium carbonate Sigma R6504-1L Prepare according the protocol for Candida albicans drug susceptibility testing
fetal bovine serum (FBS) Sigma F7524
Polyurethane tripe-lumen intravenous catheter piece (2.4 mm diameter, Certofix Trio S730) BBraun CV-15703 CV-15703
Dexamethasone Fagron SAS, France 611139 Immunosuppressant (stock solution 10 mg/ml)
Insulin syringes (0.3 ml) Terumo Myjector 29G 324826 For injection of coelenterazine
Electric razor For small animals
Sterile surgical tools Scissors, 2 pairs of tweezers, scalpel
Heating pad Leica 14042321474
Skin suture Johnson&Johnson K890H Surgical thread, needle
BLI camera (IVIS Spectrum) Perkin Elmer, Alameda IVISSPE
Living Image software Perkin Elmer, Alameda (version 4.2)

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Bu Makaleden Alıntı Yapın
Candida albicans Biofilm Formation in an In Vivo Mouse Model. J. Vis. Exp. (Pending Publication), e21797, doi: (2023).

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