Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid

Published: October 31, 2023

Abstract

Source: Trevaskis, N. L., et al. The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport. J. Vis. Exp. (2015).

This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Cannulation of the Mesenteric Lymph Duct Place the animal with its right side facing toward the operator. Perform the surgery with or without the assistance of a surgical microscope as required. Open the top layer of the abdominal muscle wall with a straight 4 cm incision extending from the midline (xiphoid pr…

Representative Results

Figure 1. Photographs of (A) the abdomen shaved in preparation to cannulate the lymph duct, (B) the abdominal muscle wall opened with a straight 4 cm incision extending from the midline to the right flank to access the lymph duct, and (C) the superior mesenteric lymph duct (in yellow circle) perpendicular to the right kidney.

Açıklamalar

The authors have nothing to disclose.

Materials

Sterile saline Baxter healthcare AHB 1307 Any brand can be used. Example here is Baxter 100 ml saline bags, box of 50
70 % ethanol in water Any Any brand can be used
Chlorhexidine gluconate solution (Microshield 4) Livingstone International JJ60243L Any brand can be used. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =JJ60243L
Betadine solution Livingstone International BU0510 Any brand can be used. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =BU0510
Ilium Ketamil (Ketamine 100 mg/ ml) PROVET VICTORIA KETA I 1 http://www.provet.vom.au
Heparin (35000I.U. in 35 mL SigmaPharmaceuticals 337220 http://www.sigmaco.com.au
Polyethylene (PE) cannula o.d. 0.96 mm x i.d. 0.58 mm Microtube extensions PE8050 Any brand can be used. Example here is PE tubing 0.96 x0.58 mm, 30 m
Polyethylene (PE) cannula o.d. 0.8
mm x i.d. 0.5 mm
Microtube extensions PE9658 Any brand can be used. Example here is PE tubing 0.8 x0.5 mm, 30 m
Markers Any Any brand can be used
Veterinary adhesive Any Any brand can be used
23 gauge needles Livingstone International DN23GX0.75LV Any brand can be used. Example here is Livingstone Disposable Needle, Sterile, 23GX0.75inch, 100/BOX. http:// www.livingstone.com.au/? PG=search_result&CAT= 6&search=DN23GX0.75LV
25 gauge needles Livingstone International DN25GX1.0LV Any brand can be used. Example here is Livingstone Disposable Needle, Sterile, 25GX1.0inch, 100/BOX. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search= DN25GX1.0LV
1 ml syringe Livingstone International T3SS01TA Any brand can be used. Example here is Terumo syringe 1 ml Slip Tuberculin 100/Box. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =T3SS01TA
10 ml syringe Livingstone International T3SS10SA Any brand can be used. Example here is Terumo syringe 10 ml Slip 100/Box. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =T3SS10SA
Gauze swabs Livingstone International GSC075 Any brand can be used and cut to required size. Example here is gauze swabs cotton filled 7.5×7.5 cm, 8 ply. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =GSC075
Heating pad Ratek WT1 Any brand that keeps temperature at 37C can be used. Example here is Ratek warming tray.
Surgical microscope Zeiss 495005-0014-000 Any brand can be used. Example here is Zeiss Stereomicroscope Stemi 2000-C with Stand S Double Spot and KL 300 LED. https:// www.micro-shop.zeiss.com/? l=en&p=us&f=e&i=10143
1 x Small surgical scissors Fine Science Tools (FST) 14060-09 Any brand can be used. Example here is FST Fine Scissors, 9 cm with 21 mm cutting edge, sharp, straight. http://www.finescience.ca/ Special-Pages/Products.aspx? ProductId=40&CategoryId=17
2 x Forceps with serrated curved tip Fine Science Tools (FST) 11001-13 Any brand can be used. Example here is FST 13 cm standard pattern forceps with curved 2.8×1.4 mm tip. http://www.finescience.ca/ Special-Pages/Products.aspx? ProductId=405&CategoryId=32

Etiketler

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Bu Makaleden Alıntı Yapın
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid. J. Vis. Exp. (Pending Publication), e21759, doi: (2023).

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