Jugular Vein Graft Harvest From a Rabbit Model: A Surgical Procedure to Excise a Segment of Rabbit Jugular Vein for Ex Vivo Studies
Published: April 30, 2023
Abstract
Source: Nishio, H., et al. A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure J. Vis. Exp. (2020).
In this video, we demonstrate a procedure for surgically harvesting a segment of the jugular vein from a rabbit model. The resected segment can be used as an autologous vein graft to study intimal hyperplasia that develops due to arterial blood pressure.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Harvest of the jugular vein NOTE: Local anesthetics (such as lidocaine) should be used before making the skin incision. Before skin incision, administer prophylactic enrofloxacin (5 mg/kg) subcutaneously. For analgesia, administer 0.05 mg/kg of buprenorphine subcutaneously twice a day for 3 d…
Açıklamalar
The authors have nothing to disclose.
Materials
| 2-0 VICRYL Plus | Johnson and Johnson | VCP316H | Surgical expendables |
| 4-0 Silk suture | Alfresa pharma | GA04SB | Surgical expendables |
| Fogarty Catheter (2Fr) | Edwards Lifesciences LLC | E-060-2F | Surgical expendables |
| 10% Povidone-iodine solution | Nakakita | 872612 | Surgical expendables |
| Intravenous catheter (20G) | Terumo | SR-OT2051C | Surgical expendables |
| Lidocaine hydrochloride | MP Biomedicals | 193917 | Anesthesia |
Etiketler
Bu Makaleden Alıntı Yapın
Jugular Vein Graft Harvest From a Rabbit Model: A Surgical Procedure to Excise a Segment of Rabbit Jugular Vein for Ex Vivo Studies. J. Vis. Exp. (Pending Publication), e20917, doi: (2023).