In this video, we evaluate the phagocytic capacity of purified astrocytes in vitro by live imaging assay. The assay serves as an effective screening platform to identify chemical compounds that can enhance or inhibit the phagocytic capacity of astrocytes.
Protocol
1. Phagocytosis Live-imaging Assay (Figure 1) Prepare a 24-well plate (or any plate/dish that is prepared for live-imaging) in which astrocytes are confluent (generally, 7 to 10 days of incubation after the purification is ideal for stabilizing the cells). Remove the media in each well and wash the wells with 1 mL of Dulbecco’s phosphate buffer saline (DPBS), three times. To minimize air exposure, wash the wells quickly. Add 300 µL of immunopann…
Representative Results
Figure 1. Schematic of phagocytosis live-imaging assay.
In Vitro Phagocytosis Assay: A Live Imaging Based Method to Study Real-time Astrocyte Mediated Synaptosome Phagocytosis. J. Vis. Exp. (Pending Publication), e20694, doi: (2023).