Intranasal Delivery of Therapeutic Neural Stem Cells (NSCs) in Murine Model: A Procedure for Tumor-targeted Delivery of Oncolytic Virus Loaded NSCs into Brain Tumor Bearing Mouse Model
This video demonstrates the intranasal delivery of oncolytic virus loaded therapeutic neural stem cells into a brain-tumor bearing mouse model. The non-invasive intranasal administration of stem cells bypasses the blood brain barrier, facilitating their delivery to the tumor site in the brain for therapeutic applications.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Hypoxic Preconditioning of Human NSCs (Figure 1A) Plate cultured NSCs in T75 culture flasks until 90% confluency is reached. Place cell culture flasks inside a humidified CO2 chamber/incubator with maintained 1% O2 levels for 24 h, via N2…
Representative Results
Figure 1: A flow chart for general stem cell preparation procedures involved prior to intranasal delivery. (A–C) Hypoxic pretreatment or genetic modifications of stem cells to overexpress CXCR4. (B–D) Washing, enzymatic dissociation, cell collection, and counting steps are performed for subsequent loading of stem cells with therapeutic or diagnostic c…
Intranasal Delivery of Therapeutic Neural Stem Cells (NSCs) in Murine Model: A Procedure for Tumor-targeted Delivery of Oncolytic Virus Loaded NSCs into Brain Tumor Bearing Mouse Model. J. Vis. Exp. (Pending Publication), e20690, doi: (2023).