In this video, we describe fluorescence molecular tomography or FMT for in vivo imaging of fluorescent protein-tagged glioblastoma xenografts in mouse models. The technique is useful for elucidating the effects of anti-tumor therapeutics at the molecular level.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1.FMT Imaging NOTE: According to the experimental aim, the iRFP-tagged glioblastoma cells can be monitored in vivo before, during, or after treatment. For imaging purpose, anesthetize animals using an isoflurane induction chamber, maintain in an imaging cassette during the scanning, and image…
Açıklamalar
The authors have nothing to disclose.
Materials
Fluorescence Tomography System
PerkinElmer
FMT 2500 XL
TrueQuant Imaging Software
Perkin Elmer
7005319
Athymic nude mice
Charles River Laboratories
Strain Code 490 (Homozygous)
Prior approval by the Institutional
Animal Care Program and by the
Institutional Biosafety Committee
required.
Fluorescence Molecular Tomography: An Imaging Technique for In Vivo Imaging of Fluorescent Protein-tagged Glioblastoma Xenografts in Mouse Model. J. Vis. Exp. (Pending Publication), e20677, doi: (2023).