This video describes a technique to harvest individual lobes from the prostate gland of a mouse model. Individual lobes can then be used for histological analysis and cancer research.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Gross Anatomy of the Prostate and Individual Lobe Microdissection NOTE: This is depicted in Figures 1(i-n) and Figure 2 Flip the tissue with a pair of blunt forceps so that the dorsal side faces up, showing the dorsal lobes, which resemble a butte…
Representative Results
Figure 1: Dissection of the mouse prostate. Step-by-step images for the dissection of the mouse prostate from the UGS: (a) removing fat, (b) removing the urinary bladder, (c) removing the vas deferens, (d) ventral view of the prostate with the urethra, (e) dorsal view of the prostate with the urethra, (f-…
Açıklamalar
The authors have nothing to disclose.
Materials
Mouse surgical instruments (Mouse Dissecting kit)
World Precision Instruments
MOUSEKIT
Dissection microscope
RPMI medium
Thermofisher Scientific
11875093
Dissection medium (DMEM + 10%FBS)
Thermofisher Scientific
11965-084
Fetal Bovine Serum
Thermofisher Scientific
10438018
PBS (Phosphate buffered saline)
Thermofisher Scientific
10010031
Collagenase
Thermofisher Scientific
17018029
Make 10x stock (10mg/ml) in RPMI, filter sterilize, aliquot and store at -20 °C
Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate. J. Vis. Exp. (Pending Publication), e20394, doi: (2023).