Determining Optimal Inhibitory Tumor Treating Field Frequency for Cancer Cells: A Non-invasive In Vitro Method to Assess the Effect of TTFields on Cancer Cell Viability

Published: April 30, 2023

Abstract

Source: Porat, Y. et al. Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields (TTFields). J. Vis. Exp. (2017)

This video demonstrates the effect of TTField application on the growth of ovarian cancer cell lines in vitro. This assay can help determine the optimal frequency of the electric field that can lead to a maximum reduction in the number of cancer cells.

Protocol

1. TTFields In Vitro Application System - Base Plate and Dish Maintenance Rinse dishes and dish covers under running tap water. Then, rinse with deionized water and air-dry face down. If a chemotherapeutic agent/drug has been added to dishes in a prior experiment, fill each dish with a 5% light detergent solution and leave overnight. Rinse the dishes thoroughly under running tap water to avoid any traces of detergent inside the dish. Rinse the dishes and di…

Açıklamalar

The authors have nothing to disclose.

Materials

Invitro system and software Novocure ITG1000 and IBP1000 Each unit contains 1 TTFields generator, 1 base plate, 8 TTFields dishes with covers and 1 flat cable.
Sterilization bags Westfield medica 24882
Plastic cover slides Thermo Scientific (NUNC) 174977 Pre treated and sterilized
Glass cover slides Thermo Scientific (Menzel-Gläser) CB00220RA1 Sterilize if necessary
Dulbecco’s modified Eagle’s medium Biological Industries (Israel) 01-055-1A Warm in 37 °C water bath before use
RPMI 1640 Gibco 21875-034 Warm in 37 °C water bath before use
Fetal Bovine Serum (FBS) Biological Industries (Israel) 04-007-1A Warm in 37 °C water bath before use
L-Glutamine 200 mM (100x) Gibco 25030-029
Pen/Strep (10,000 U/mL Penicillin, 10,000 µg/mL Streptomycin) Gibco 15140-122
Sodium Pyruvate solution 100 mM Biological Industries (Israel) 03-042-1B
Hepes buffer 1 M Biological Industries (Israel) 03-025-1B
Insulin solution from bovine pancreas Sigma-Aldrich 10516-5ML
0.25% Trypsin/EDTA Biological Industries (Israel) 03-050-1B Warm in 37 °C water bath before use
Methanol Merck 1.06009.2511 Cool to -20 °C in the freezer before use
PBS Biological Industries (Israel) 02-023-1A Without calcium and magnesium
A2780 ECACC 93112519 Grow in RPMI 1640 supplemented with FBS (10%), pen/strep (100 U/ mL / 100 µg/mL), sodium pyruvate (1 mM) and Hepes buffer (12 mM).
F98 ATCC CRL-2397 Grow in Dulbecco’s modified Eagle’s medium supplemented with FBS (10%), pen/strep (100 U/mL / 100 µg/mL), sodium pyruvate
(1 mM) and glutamine (2 mM).
Ovcar-3 ATCC HTB-161 Grow in RPMI 1640 supplemented with FBS (20%), pen/strep (100 U/ mL / 100 µg/mL), sodium pyruvate (1 mM), Hepes buffer (12 mM) and insulin (10 µg/mL).
U-87 MG ATCC HTB-14 Grow in Dulbecco’s modified Eagle’s medium supplemented with FBS (10%), pen/strep (100 U/mL / 100 µg/mL), sodium pyruvate (1 mM) and glutamine (2 mM).
refrigirated CO2 incubator CARON 7404-10-3
Laminar flow cabinet ADS Laminair Bio12 and VSM12

Etiketler

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Bu Makaleden Alıntı Yapın
Determining Optimal Inhibitory Tumor Treating Field Frequency for Cancer Cells: A Non-invasive In Vitro Method to Assess the Effect of TTFields on Cancer Cell Viability. J. Vis. Exp. (Pending Publication), e20384, doi: (2023).

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