Source: Amaro-Ortiz, A. et al. Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model. J. Vis. Exp. (2013)
This video describes a method to measure UV sensitivity in a humanized mouse model—a mouse model that mimics human skin. We determine the minimal erythematous dose (MED) of UV radiation that can cause erythema or edema on the skin. The method can help to find out the efficacy of drugs used to reduce UV sensitivity.
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
Determination of UV Sensitivity by Calculation of "Minimal Erythematous Dose" (MED)
Figure 1. Forskolin-induced melanization protects against UV-mediated inflammation as determined by minimal erythematous dose (MED) testing. (A,B) Position of UV occlusive tape and UVB doses of animals treated twice daily for 5 days (A,C) or once daily for 15 days (B,D,E) with either vehicle or forskolin. The last topical treatment was applied 48 hr prior to irradiation. Dorsal skin was exposed to various doses of UVB by using UV-occlusive tape with punched-out 1 cm2 circular apertures, and varying exposure times to yield the appropriate dose. After irradiation, circles of exposed skin were labeled with a pen in some experiments. MED's, defined by erythema and/or edema of the entire circle of exposed skin to a particular dose, were determined 48 hr after exposure. The MED ± SD results are reported as kJ/m2 UVB, * p≤0.001. (E) Skin color reflectometry and MED values for tyrosine-deficient K14-Scf albino extension mice treated for 10 days with vehicle
The authors have nothing to disclose.
Xylazine | Anased Injection | LA04612 | Shenandoah, Iowa, USA |
Ketamine HCl, USP | Putney | NDC 26637-411-01 | St. Joseph, MO, USA |
Ethanol | Decon Labs. | 2705 | |
Depilatory cream, Nair | Church Dwight | JF-11 4381322 | Priceton, NJ |
Germicidal Hg Lamp UV-B | Westinghouse | F15T8UV-B | |
Radiometer photometer | International light | 1LT400A | Peabody, MA,USA |
Isothesia, Isoflurane , USP | Butler Schein | NCD 11695-6776-1 | Dublin, OH, USA |
Chromameter | Konica Minolta | CR-400 | Ramsey, NJ, USA |
Data Processor for Chromameter CR-400 | Konica Minolta | DR-400 | Ramsey, NJ, USA |