Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients

Published: April 30, 2023

Abstract

Source: Giallombardo, M., et al. Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool. J. Vis. Exp. (2016).

The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.

Protocol

1. Exosome Isolation Note: The isolation of exosomes from plasma samples, was performed with a commercial kit, according to manufacturer's protocol and by adding RNAse treatment: (all these steps must be performed with personal and environment protective equipments and following specific legal procedure for manipulation of biological samples). Take 1 ml of plasma of each sample, stored at -80 °C, and place it on ice. Centrifuge the sample at 2,00…

Açıklamalar

The authors have nothing to disclose.

Materials

Total exosome isolation kit (from plasma) Invitrogen 4484450 Use Proteinase K treatment
Ribonuclease A Sigma-Aldrich R4875-100MG
RNAspin Illustra mini kit 50 GE HealthCare 25-0500-71

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Bu Makaleden Alıntı Yapın
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients. J. Vis. Exp. (Pending Publication), e20250, doi: (2023).

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