JOVE JOURNAL

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Journal (Neuroscience)

Generation of Human Retinal Organoids and Co-Culturing with Microglia for Disease Modeling and Drug Screening

Jia Xu*¹, Si-Jian Yu*¹, Zi-Bing Jin

¹Beijing Institute of Ophthalmology, Beijing Tongren Hospital,Capital Medical University

Journal (Neuroscience)

Assembling Retinal Organoids with Microglia

Jia Xu*¹, Si-Jian Yu*¹, Zi-Bing Jin

¹Beijing Institute of Ophthalmology, Beijing Tongren Hospital,Capital Medical University

Journal (Neuroscience)

Isolating Immune Cells from Mouse Brain and Skull

Ran Zhang, Jin Zhang, Ata Ur Rehman, Lihong Dang, Xinyuan Yu, Wei Yang

¹Multidisciplinary Brain Protection Program (MBPP), Department of Anesthesiology,Duke University Medical Center

Journal (Neuroscience)

Preparing Single-Cell Suspension from Mouse Brain to Study Immune Response to Brain Disorders

Ran Zhang, Jin Zhang, Ata Ur Rehman, Lihong Dang, Xinyuan Yu, Wei Yang

¹Multidisciplinary Brain Protection Program (MBPP), Department of Anesthesiology,Duke University Medical Center

Journal (Neuroscience)

Preparing Bone Marrow Single-Cell Suspension from Mouse Calvaria to Study Immune Response to Brain Disorders

Ran Zhang, Jin Zhang, Ata Ur Rehman, Lihong Dang, Xinyuan Yu, Wei Yang

¹Multidisciplinary Brain Protection Program (MBPP), Department of Anesthesiology,Duke University Medical Center

Journal (Neuroscience)

Targeted Brain Region Microinjection of Bromophenol Dye in Mice

Xiaoxin Zhou*^1,2,3, Wanbing Dai*^1,2, Jie Zhou*^1,2, Yizhe Zhang², Xiao Zhang², Sihan Chen², Xiaoyu Sun²

¹Department of Anesthesiology, Renji Hospital,Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Shanghai General Hospital,Shanghai Jiaotong University

Journal (Neuroscience)

Brain Tissue Harvesting, Cryosectioning, and Imaging Post Stereotaxic Surgery in Mice

Xiaoxin Zhou*^1,2,3, Wanbing Dai*^1,2, Jie Zhou*^1,2, Yizhe Zhang², Xiao Zhang², Sihan Chen², Xiaoyu Sun²

¹Department of Anesthesiology, Renji Hospital,Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Shanghai General Hospital,Shanghai Jiaotong University

Journal (Neuroscience)

Preliminary Validation of Stereotaxic Injection Coordinates via Cryosectioning

Xiaoxin Zhou*^1,2,3, Wanbing Dai*^1,2, Jie Zhou*^1,2, Yizhe Zhang², Xiao Zhang², Sihan Chen², Xiaoyu Sun²

¹Department of Anesthesiology, Renji Hospital,Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Shanghai General Hospital,Shanghai Jiaotong University

Journal (Neuroscience)

Analysis of Immune Cell Diversity Using Histo-Flow Cytometry

Rajiv W. Jain², David A. Elliott, V. Wee Yong²

¹Hotchkiss Brain Institute,University of Calgary, ²Department of Clinical Neurosciences,University of Calgary

Journal (Neuroscience)

Generating and Analyzing High-Parameter Histology Images with Histoflow Cytometry

Rajiv W. Jain², David A. Elliott, V. Wee Yong²

¹Hotchkiss Brain Institute,University of Calgary, ²Department of Clinical Neurosciences,University of Calgary

Journal (Neuroscience)

Retinal Organoid Fixation and Post Fixation Procedure for TEM Sample Preparation

Xiaoqing Liu*^1,2, Bilin Rao*^1,2, Qingyang Lin², Meiling Gao², Jun Zhang²

¹State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital,Wenzhou Medical University, ²Laboratory of Retinal Physiology and Disease, School of Ophthalmology and Optometry,Wenzhou Medical University

Journal (Neuroscience)

Preparing Retinal Organoid Samples for Transmission Electron Microscopy

Xiaoqing Liu*^1,2, Bilin Rao*^1,2, Qingyang Lin², Meiling Gao², Jun Zhang²

¹State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital,Wenzhou Medical University, ²Laboratory of Retinal Physiology and Disease, School of Ophthalmology and Optometry,Wenzhou Medical University

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