JOVE JOURNAL

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Generation of Human Brain Organoids for Mitochondrial Disease Modeling

Stephanie Le, Laura Petersilie, Gizem Inak⁴, Carmen Menacho-Pando, Karl W. Kafitz, Agnieszka Rybak-Wolf, Nikolaus Rajewsky, Christine R. Rose, Alessandro Prigione⁵

¹Department of General Pediatrics, Neonatology and Pediatric Cardiology, Duesseldorf University Hospital, Medical Faculty,Heinrich Heine University, ²Institute of Neurobiology,Heinrich Heine University, ³Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrueck Center for Molecular Medicine (MDC), ⁴Seaver Autism Center for Research and Treatment,Icahn School of Medicine at Mount Sinai, ⁵Max Delbrueck Center for Molecular Medicine (MDC)

Journal (Neuroscience)

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

Jesper Peter Bömers², Sara Ellinor Johansson, Lars Edvinsson⁴, Tiit Illimar Mathiesen^3,5, Kristian Agmund Haanes

¹Department of Neurosurgery,Rigshospitalet, ²Department of Clinical Experimental Research,Glostrup Research Institute, Rigshospitalet, ³Department of Clinical Medicine,University of Copenhagen, ⁴Department of Clinical Sciences, Division of Experimental Vascular Research,Lund University, ⁵Department of Clinical Neuroscience,Karolinska Institutet

Journal (Neuroscience)

In vivo Calcium Imaging in Mouse Inferior Olive

Da Guo, Ayşen Gürkan Özer, Marylka Yoe Uusisaari

¹Neuronal Rhythms in Movement unit,OIST

Journal (Neuroscience)

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Cassandra N. Dennys, Julieth A. Sierra-Delgado, Shrestha Sinha Ray, Annalisa M. Hartlaub, Florence S. Roussel, Yacidzohara Rodriguez, Kathrin Meyer²

¹Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, ²College of Medicine, The Ohio State University

Journal (Neuroscience)

Direct Cannula Implantation in the Cisterna Magna of Pigs

Nicholas B. Bèchet², Nagesh C. Shanbhag², Iben Lundgaard²

¹Department of Experimental Medical Science,Lund University, ²Wallenberg Centre for Molecular Medicine,Lund University

Journal (Neuroscience)

Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development

Urann Chan, Diwas Gautam, Anne E. West

¹Department of Neurobiology,Duke University Medical Center

Journal (Neuroscience)

Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice

Yi-Tse Hsiao, Angela Yu-Chi Wang, Ting-Yen Lee, Ching-Yuan Chang

¹Department of Veterinary Medicine, School of Veterinary Medicine,National Taiwan University

Journal (Neuroscience)

A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model

Yi-Min Kuo, Yu-Yo Sun, Chia-Yi Kuan

¹Department of Anesthesiology, Taipei Veterans General Hospital,National Yang-Ming University School of Medicine, ²Department of Neuroscience, Center for Brain Immunology and Glia (BIG),University of Virginia School of Medicine

Journal (Neuroscience)

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

Faraz Ahmad, Ping Liu

¹Department of Anatomy, School of Biomedical Sciences,University of Otago

Journal (Neuroscience)

Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound

Axel Neulen*¹, Michael Molitor*^2,3,4, Michael Kosterhon*¹, Tobias Pantel, Susanne H. Karbach^3,4, Philip Wenzel^3,4, Thomas Gaul, Florian Ringel, Serge C. Thal

¹Department of Neurosurgery,University Medical Center of the Johannes Gutenberg-University of Mainz, ²Center for Cardiology - Cardiology I,University Medical Center of the Johannes Gutenberg-University of Mainz, ³Center for Thrombosis and Hemostasis (CTH),University Medical Center of the Johannes Gutenberg-University of Mainz, ⁴German Center for Cardiovascular Research (DZHK) - Partner site Rhine-Main, ⁵Department of Anesthesiology,University Medical Center of the Johannes Gutenberg-University of Mainz

Journal (Neuroscience)

A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish

James Hentig^2,3, Kaylee Cloghessy^2,3, Chloe Dunseath³, David R. Hyde^2,3

¹Department of Biological Sciences,Notre Dame Üniversitesi, ²Center for Zebrafish Research,Notre Dame Üniversitesi, ³Center for Stem Cells and Regenerative Medicine,Notre Dame Üniversitesi

Journal (Neuroscience)

Massive Pontine Hemorrhage by Dual Injection of Autologous Blood

Xiangyue Tang*¹, Li Wu*¹, Ming Luo, Zhihua Qiu, Yongjun Jiang

¹The Second Affiliated Hospital of Guangzhou Medical University

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