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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Sara Tognarelli*^1,2, Benedikt Jacobs*^3,4, Nina Staiger^1,2, Evelyn Ullrich^1,2

¹Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology,Johann Wolfgang Goethe-University, ²LOEWE Center for Cell and Gene Therapy,Johann Wolfgang Goethe-University, ³Institute for Cancer Research, Department of Cancer Immunology,Oslo University Hospital, Radiumhospital, ⁴The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine,University of Oslo

Journal (Immunology and Infection)

Isolation and Activation of Murine Lymphocytes

Jun Feng Lim¹, Heidi Berger¹, I-hsin Su¹

¹School of Biological Sciences, College of Science,Nanyang Technological University

Journal (Immunology and Infection)

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Ming Yuan*¹, Pengju Wang*^2,3, Louisa S. Chard¹, Nicholas R. Lemoine^1,2,3, Yaohe Wang^1,2,3

¹Center for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London, ²Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy,Zhengzhou University, ³School of Basic Medical Sciences, Academy of Medical Sciences,Zhengzhou University

Journal (Immunology and Infection)

Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies

Rajiv W. Jain¹, Amy K. Dang¹, Steven M. Kerfoot¹

¹Department of Microbiology and Immunology,Western University

Journal (Immunology and Infection)

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Tingxi Guo^1,2, Toshiki Ochi*², Munehide Nakatsugawa*², Yuki Kagoya², Mark Anczurowski^1,2, Chung-Hsi Wang^1,2, Muhammed A. Rahman², Kayoko Saso², Marcus O. Butler^1,2, Naoto Hirano^1,2

¹Department of Immunology,University of Toronto, ²Princess Margaret Cancer Centre,University Health Network

Journal (Immunology and Infection)

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

Danae Schulz^1,2, Monica R. Mugnier¹, Catherine E. Boothroyd^1,3, F. Nina Papavasiliou¹

¹Laboratory of Lymphocyte Biology,Rockefeller University, ²Biyoloji Bölümü,Harvey Mudd College, ³Masters School

Journal (Immunology and Infection)

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

Salome Murinello¹, Stacey K. Moreno¹, Matthew S. Macauley², Susumu Sakimoto¹, Peter D. Westenskow^1,3, Martin Friedlander¹

¹Department of Cell and Molecular Biology,The Scripps Research Institute, ²Department of Chemical Physiology,The Scripps Research Institute, ³The Lowy Medical Research Institute

Journal (Immunology and Infection)

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Alfonso Rubio-Navarro¹, Melania Guerrero-Hue¹, Beatriz Martín-Fernandez², Isabel Cortegano³, Elena Olivares-Alvaro², Natalia de las Heras², Mario Alía³, Belén de Andrés³, María Luisa Gaspar³, Jesús Egido¹, Juan Antonio Moreno¹

¹Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz,Autonoma University, ²Department of Physiology, Faculty of Medicine,Complutense University, ³Department of Immunology, Centro Nacional de Microbiologìa,Instituto de Salud Carlos III (ISCIII)

Journal (Immunology and Infection)

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Gloria Figueroa¹, Tiyash Parira¹, Alejandra Laverde¹, Gianna Casteleiro¹, Amal El-Mabhouh², Madhavan Nair¹, Marisela Agudelo¹

¹Department of Immunology, Herbert Wertheim College of Medicine,Florida International University, ²Millipore Sigma

Journal (Immunology and Infection)

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

Vishnu Priya Bollampalli¹, Susanne Nylén¹, Antonio Gigliotti Rothfuchs¹

¹Department of Microbiology, Tumor and Cell Biology (MTC),Karolinska Institutet

Journal (Immunology and Infection)

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Tabitha Bucher¹, Elena Kartvelishvily², Ilana Kolodkin-Gal¹

¹Department of Molecular Genetics,Weizmann Institute of Science, ²Electron Microscopy Unit,Weizmann Institute of Science

Journal (Immunology and Infection)

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices

Steven Mazur¹, Michael R. Holbrook¹, Tracey Burdette¹, Nicole Josleyn¹, Jason Barr¹, Daniela Pusl¹, Laura Bollinger¹, Linda Coe¹, Peter B. Jahrling¹, Matthew G. Lackemeyer¹, Jiro Wada¹, Jens H. Kuhn¹, Krisztina Janosko¹

¹Integrated Research Facility at Frederick, National Institute of Allergy and Infectious Diseases (NIAID),National Institutes of Health (NIH)

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