JOVE JOURNAL

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Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Joselyn N. Allen¹, Adwitia Dey¹, Ruth Nissly², James Fraser¹, Shan Yu¹, Gayathri Balandaram¹, Jeffrey M. Peters¹, Pamela A. Hankey-Giblin¹

¹Department of Veterinary and Biomedical Sciences,The Pennsylvania State University, ²Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences,Pennsylvania State University

Journal (Immunology and Infection)

Increased Recovery Time and Decreased LPS Administration to Study the Vagus Nerve Stimulation Mechanisms in Limited Inflammatory Responses

Erwan Le Maître¹, Priya Revathikumar¹, Johanna Estelius¹, Jon Lampa¹

¹Department of Medicine, Unit of Rheumatology, Center for Molecular Medicine (CMM), Karolinska Institute,Karolinska University Hospital

Journal (Immunology and Infection)

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

Graham Brogden*^1,2, Ariane Neumann*^1,3, Diab M. Husein¹, Friederike Reuner^1,4, Hassan Y. Naim¹, Maren von Köckritz-Blickwede^1,4

¹Department of Physiological Chemistry,University of Veterinary Medicine Hannover, ²Fish Disease Research Unit,University of Veterinary Medicine, ³Department of Clinical Sciences, Biomedical Center,Lund University, ⁴Research Center for Emerging Infections and Zoonoses (RIZ),University of Veterinary Medicine Hannover

Journal (Immunology and Infection)

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

Kaitlyn Schaaf¹, Samuel R. Smith¹, Virginia Hayley¹, Olaf Kutsch¹, Jim Sun¹

¹Tıp Fakültesi,University of Alabama at Birmingham

Journal (Immunology and Infection)

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Chien-Wen Chen¹, Chi-Yao Chang¹

¹Institute of Cellular and Organismic Biology,Academia Sinica

Journal (Immunology and Infection)

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Dorothee Weihrauch¹, John G. Krolikowski^1,2, Deron W. Jones³, Tahniyath Zaman³, Omoshalewa Bamkole¹, Janine Struve⁴, Paul S. Pagel⁵, Nicole L. Lohr⁶, Kirkwood A. Pritchard, Jr.³

¹Department of Anesthesiology,Medical College of Wisconsin, ²Clement J. Zablocki Veterans Affairs Medical Center, ³Department of Surgery, Division of Pediatric Surgery,Children's Research Institute, ⁴Department of Orthopedic Surgery,Medical College of Wisconsin, ⁵Deptarment of Anesthesiology,Clement J Zblocki Veteran Affairs Medical Center, ⁶Department of Medicine, Division of Cardiology,Medical College of Wisconsin

Journal (Immunology and Infection)

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Jennifer E. McBride¹, Mary P. Meaney², Casey John², David C. Nieman², Renaud F. Warin¹

¹Center for Excellence in Post-Harvest Technologies,North Carolina A&T State University, North Carolina Research Campus, ²Human Performance Laboratory,Appalachian State University, North Carolina Research Campus

Journal (Immunology and Infection)

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Brian Czaya*^1,2, Saurav Singh*^1,2, Christopher Yanucil^1,2, Karla Schramm^1,2, Christian Faul^1,2, Alexander Grabner¹

¹Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, ²Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Journal (Immunology and Infection)

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Ahmed Fouda¹, Mahasti Tahsini², Fatemeh Khodayarian², Fatimah Al-nafisah², Moutih Rafei^1,2,3

¹Department of Microbiology, Infectiology and Immunology,Université de Montréal, ²Department of Pharmacology and Physiology,Université de Montréal, ³Molecular Biology Program,Université de Montréal

Journal (Immunology and Infection)

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Fabio Cafini*^1,2, Nguyen Thi Le Thuy*³, Federico Román⁴, José Prieto⁵, Sarah Dubrac^6,7, Tarek Msadek^6,7, Kazuya Morikawa¹

¹Division of Biomedical Science, Faculty of Medicine,University of Tsukuba, ²Department of Basic Biomedical Science,Universidad Europea de Madrid, ³Human Biology Program, School of Integrative and Global Majors,University of Tsukuba, ⁴Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa,Instituto de Salud Carlos III, ⁵Division of Microbiology, Department of Medicine, School of Medicine,Universidad Complutense, ⁶Biology of Gram-Positive Pathogens, Department of Microbiology,Institut Pasteur, Paris, France, ⁷ERL3526,CNRS, Paris, France

Journal (Immunology and Infection)

Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples

Anne Mayer-Scholl¹, Edoardo Pozio², Jennifer Gayda¹, Nora Thaben¹, Peter Bahn¹, Karsten Nöckler¹

¹Federal Institute for Risk Assessment, ²Istituto Superiore di Sanità

Journal (Immunology and Infection)

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Collins Cheruiyot¹, Zemplen Pataki¹, Robert Williams¹, Bharat Ramratnam^2,3, Ming Li³

¹Brown University, ²COBRE Center for Cancer Research, Lifespan Laboratories,Rhode Island and Miriam Hospitals, ³Division of Infectious Diseases, Department of Medicine,Warren Alpert Medical School, Brown University

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