JOVE JOURNAL

İmmünoloji ve Enfeksiyon

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Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Irati Romero-Garmendia*¹, Amaia Jauregi-Miguel*¹, Izortze Santin², Jose Ramón Bilbao¹, Ainara Castellanos-Rubio¹

¹Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, ²Endocrinology and Diabetes Research Group, Biocruces Health Research Institute,UPV-EHU, CIBERDEM

Journal (Immunology and Infection)

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Dhrubajyoti Nag¹, Kristie Mitchell¹, Paul Breen¹, Jeffrey H. Withey¹

¹Department of Microbiology, Immunology, and Biochemistry,Wayne Eyalet Üniversitesi Tıp Fakültesi

Journal (Immunology and Infection)

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Ron Balczon^1,4, Michael Francis^2,4, Silas Leavesley^3,4, Troy Stevens^2,4

¹Biyokimya ve Moleküler Biyoloji Anabilim Dalı,University of South Alabama, ²Department of Physiology and Cell Biology,University of South Alabama, ³Department of Chemical and Biomolecular Engineering,University of South Alabama, ⁴Center for Lung Biology,University of South Alabama

Journal (Immunology and Infection)

A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

Effie E Bastounis¹, Fabian E Ortega¹, Ricardo Serrano², Julie A Theriot³

¹Department of Biochemistry,Stanford University School of Medicine, ²Department of Mechanical and Aerospace Engineering,University of California San Diego, ³Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute,Stanford University School of Medicine

Journal (Immunology and Infection)

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

Volodymyr Tsvilovskyy¹, Alejandra Solis-Lopez¹, Kathrin Öhlenschläger¹, Marc Freichel¹

¹Institute of Pharmacology,Ruprecht-Karls Heidelberg University

Journal (Immunology and Infection)

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

Quinton L. Anderson¹, Alexey V. Revtovich¹, Natalia V. Kirienko¹

¹Department of Biosciences,Rice University

Journal (Immunology and Infection)

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Shouming Zhang^1,2,3, Xueping Gao^1,2,3, Genhui Xiao^1,2,3,4, Chengping Lu^1,2,3, Huochun Yao^1,2,3, Hongjie Fan^1,2,3,5, Zongfu Wu^1,2,3

¹College of Veterinary Medicine,Nanjing Agricultural University, ²Key Lab of Animal Bacteriology,Ministry of Agriculture, ³OIE Reference Lab for Swine Streptococcosis, ⁴School of Medicine,Tsinghua University, ⁵Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Journal (Immunology and Infection)

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Maiken W. Rosenstierne¹, Christopher E. Jensen¹, Anders Fomsgaard^1,2

¹Virus Research & Development Laboratory,Statens Serum Institut, ²Infectious Disease Research Unit,University of Southern Denmark

Journal (Immunology and Infection)

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Ryan M. Baxter*¹, Daniel S. Kong*¹, Josselyn E. Garcia-Perez¹, William E. O'Gorman², Elena W.Y. Hsieh^1,3

¹Department of Immunology and Microbiology,University of Colorado School of Medicine, ²OMNI Biomarkers, Development Sciences,Genentech, ³Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Journal (Immunology and Infection)

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

Jingshu Chen*², Lei Zhong*¹, Jing Wu¹, Sui Ke², Benjamin Morpurgo³, Andrei Golovko³, Nengtai Ouyang⁴, Yuxiang Sun², Shaodong Guo², Yanan Tian^1,2

¹Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization,Hunan Agriculture University, ²Texas A&M University, ³Texas A&M Institute for Genomic Medicine, ⁴Sun Yat-Sen Memorial Hospital

Journal (Immunology and Infection)

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Ola H. Negm^1,2, Mohamed Hamed^1,2, Tanya M. Monaghan³

¹Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre,University of Nottingham, ²Medical Microbiology and Immunology Department, Faculty of Medicine,Mansoura University, ³Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre,University of Nottingham

Journal (Immunology and Infection)

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

Jordan F. Hastings¹, Jeremy Z.R. Han¹, Robert F. Shearer^1,2, Sean P. Kennedy^1,3, Mary Iconomou^1,4, Darren N. Saunders⁵, David R. Croucher^1,6,7

¹The Kinghorn Cancer Centre,Garvan Institute of Medical Research, ²Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences,University of Copenhagen, ³RCSI Molecular Medicine,Royal College of Surgeons in Ireland, ⁴Department of Epigenetics,Max Planck Institute of Immunobiology and Epigenetics, ⁵School of Medical Sciences,University of New South Wales, ⁶St Vincent's Hospital Clinical School,University of New South Wales, ⁷School of Medicine and Medical Science,University College Dublin

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